فراوانی آلل‌ها و ژنوتایپ‌های ApaI ژن رسپتور ویتامین D در زنان آذری ایرانی با بیماری سندرم تخمدان پلی‌کیستیک و کنترل سالم

  Received: 19 Oct, 2012 Accepted: 22 Dec, 2012     Background & Aims : Polycystic Ovary Syndrome (PCOS) as a heterogeneous disease is one of the most usual women endocrine disorders and the leading causes of infertility by reason of unovulation. PCOS affecting about between 5 and 10 percent of females. The results of recent studies indicated that vitamin D has an important role in calcium homeostasis. Many investigators imply that it is necessary to study the influence of calcium and vitamin D consumption on ovulation especially in patients with PCOS. The goal of this investigation was to determine vitamin D receptor gene ApaI alleles and genotypes frequency in Iranian Azeri women with polycystic ovary syndrome and healthy controls.   Methods & Materials: Total of 76 Iranian Azeri women including 38 women with polycystic ovary syndrome and 38 healthy controls entered at present study. RFLP-PCR method was performed for alleles and genotypes detection.   Results : The frequencies of VDR ApaI were 0. 67 and 0. 33 in PCOS women, also 0. 62 and 0. 38 in healthy controls regarding A and C alleles. The allelic frequencies (%frequencies) of VDR ApaI were 51(67. 11), and 25(32. 89) in PCOS women, also 47(61. 84), and 29(38. 16) in controls regarding A and C alleles, respectively. Also, the genotypic frequencies(%frequencies) of VDR ApaI were 15(39. 47), 21(55. 26), and 2(5. 26) in PCOS women, also 12(31. 58), 23(60. 53), and 3(7. 89) in healthy controls regarding AA,AC,and CC genotypes, respectively. There was no statistically significant differences in VDR ApaI allele and genotype frequencies between studied groups (p-value >0. 05).   Conclusion : The findings of present study for the first time in Iranian Azeri PCOS women imply that no association was found between the VDR ApaI and PCOS susceptibility.           SOURCE: URMIA MED J 2012: 23(7): 820 ISSN: 1027-3727